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12th Biology Weekly Test - 4 ( Zoology - Applications of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Weekly Test - 4 ( Zoology - Applications of Biotechnology )-Aug 2020

Time : 1.00 Hours

Part - A

Multiple Choice Question - Answer All Question

Throgh PCR technique, amplication of__________ is possible with reverse transcriptase.

DNA
rRNA
tRNA
mRNA

Interferons are produced using ___________

Pigs
yeast
E.coli
viruses

The genetic defect adenosine deaminase deficiency may be cured permanently by

Enzyme replacement therapy
periodic infusion of genetically engineered lymphocytes having ADA cDNA
administering adenosine deaminase activators
introducing bone marrow cells producing ADA into embryo at an early stage of development.

The genes for the formation of the blood clotting factor VIII is located in the ______________ chromosome.

autosome
X chromosome
allosome
Y chromosome

This is not the advantage of new generation vaccine.

They trigger immune response only against not specific pathogens.
They produce target proteins
They produce long lasting immunity
They are with less toxic effect.

The two chains of Insulin molecule are attached by _______

Covalent bonds
disulphide bonds
hydrogen bonds
both a and b

Transgenic animals are those which have

Foreign DNA in some of their cells
Foreign DNA in all their cells
Foreign RNA in some of their cells
Foreign RNA in all their cells

Rearrange the following steps as PCP in correct order.

1. Extension
2. Annealing
3. Denaturation

Annealing \(\rightarrow \) Extension \(\rightarrow \) Denaturation
Denaturation \(\rightarrow \) Annealing \(\rightarrow \) Extension
Annealing \(\rightarrow \) Denaturation\(\rightarrow \) Extension
Extension \(\rightarrow \) Annealing \(\rightarrow \) Denaturation

Which one of the following statements is true regarding DNA polymerase used in PCR?

It is used to ligate introduced DNA in recipient cells
It serves as a selectable marker
It is isolated from a Virus
d) It remains active at a high temperature

In the gene therapy far SCID,___________cells are removed from the blood, and ADA gene is introduced into it using retrovirus.

RBC
Wobe
Lymphocytes
monocytes

The purpose of using enzyme in the ELISA test is

to show the coloured product
to show the antigen-antibody reaction
to show the immobilised antigen
to show the immobilized antigen

Best and Banting isolated insulin from pancreatic islets.of _____________

E.coli
yeast
dog
pig

The first clinical gene therapy was done for the treatment of

AIDS
Cancer
Cystic fibrosis
SCID

If a recessive gene is involved in causin genetic disease, what is the strategy to be u in gene therapy in order to cure the disease

Injecting DNA into the genome
Injecting DNA and antisense gene
Injecting antisense gene
Removal of recessive gene

The first synthetic vaccine produced was ________

DPT
HbsAg
Measles
Polio

All the following are the functions of insulin. Except.

Insulin controls the level of glucose in the blood.
Insulin facilitates the cellular uptake of glucose.
Insulin facilitates the utilization of glucose.
Insulin breaks the glucose which guess energy.

Using recombinant E.Coli, mass production of hGH is carried out by ________

genetic engineering
biotechnology
fermentation technology
recombinant DNA

Insulin was first isolated by ________

Banting
Allen
Wilmut
Anderson

Early detection of the disease is not possible using their conventional diagnostic method.

microscopic examination
recombinant DNA technology
polymerase chain reactions
enzyme linked immunosorbent assay

The first clinical gene therapy was done for the treatment of

AIDS
Cancer
Cystic fibrosis
SCID

Choose the option not applicable to ELISA,

It is a bio chemical produce to detect antigens in a sample.
The intensity of colour obtained in the test is proportional to amount of antigen.
It can detect DNA
ELISA is a test for detecting human chorionic gonadotropin hormone

Vaccines that use components of a pathogenic organism rather than the whole organism are called

Subunit recombinant vaccines
attenuated recombinant vaccines
DNA vaccines
conventional vaccines

This is a live vaccine, in this genetically modified pathogens are made to non pathogenic.

Attenuated recombinant vaccine
Subunit recombinant vaccine
DNA vaccine
Traditional vaccine

ELISA is mainly used for

Detection of mutations
Detection of pathogens
Selecting animals having desired traits
Selecting plants having desired traits

This type of gene therapy involves the insulin C the anti sense gene which inhibits the expression of the dominant gene.

gene inhibition therapy
gene augmentation therapy
germ line gene therapy
somatic cell gene therapy

The edible subunit vaccine used against this disease is.

measles
hepatitis
cholera
feet and mouths

Alpha lactalbumin is a protein with ______ aminoacids

120
133
123
140

Interferons were discovered by __________

Banting
Flemming
Engvall
Alick Isaacs and Lindemann

Stem cells that can differentiate into various types of cells that are related in an organism is called

Multipotent
Oligopotent
Pluripotent
Unipotent

Pre-pro insulin is devoid of __________

C chain
disulphide bonds
COOH and NH₂ group
Polypeptide chain

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