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12th Biology Weekly Test - 4 ( Botany - Principles and Processes of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Weekly Test - 4 ( Botany - Principles and Processes of Biotechnology )-Aug 2020

Time : 1.00 Hours

Part - A

Multiple Choice Question - Answer All Question

T₁ plasmid does not have this gene.

Onc
tra
ori
inc

An antibiotic gene in a vector usually helps in the selection of

Competent cells
Transformed cells
Recombinant cells
None of the above

EcoRI cleaves DNA at

AGGGTT
GTATATC
GAATTC
TATAGC

Which of the following one is used as a Biosensors?

Electrophoresis
Bioreactors
Vectors
Electroporation

The term biotechnology was coined by ___________

Gottlieb
W. Bateson
Karl Ereky
Erich

Pick the characteristic of a vector which is not true.

Small size
Suitable marker
Origin of replication
One restriction site only

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction
enzymes?

5' CGTTCG 3' 3' ATCGTA 5'
5' GATATG 3' 3' CTACTA 5'
5' GAATTC 3' 3' CTTAAG 5'
5' CACGTA 3' 3' CTCAOT 5'

____________ used as biofertiliser and nitrogen fixers.

Enzymes
Biomass
Microbial inoculants
Biofuels

Restriction enzymes are

Not always required in genetic engineering
Essential tools in genetic engineering
Nucleases that cleave DNA at specific sites
both b and c

EcoRI cleaves DNA at

AGGGTT
GTATATC
GAATTC
TATAGC

Production of monoclonal antibodies by

Sanger and Gilbert
Kohler and Milstein
Watson and Crick
Karl Ereky

Process that increases oxygen flow to accelerate the degradation of environmental pollutants is __________

Mycoremediation
Bioventing
Phytoremediation
Rhizofiltration

The cry toxins effect the ___________ system of insect.

respiratory
digestive
circulatory
immune

Plasmids are

circular protein molecules
required by bacteria
tiny bacteria
confer resistance to antibiotics

Which one of the following is not correct statement

Ti plasmid causes the bunchy top disease
Multiple cloning site is known as Polylinker
Non viral method transfection of Nucleic acid in cell
Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

Plasmids are

circular protein molecules
required by bacteria
tiny bacteria
confer resistance to antibiotics

The process of recombinant DNA technology has the following steps

I. Amplification of the gene.
II. Insertion of recombinant DNA into the host cells.
III. Cutting of DNA at specific location using restriction enzyme.
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.

II, III, IV, I
IV, II, III, I
I, II, III, IV
IV, III, I, II

Some of the characteristics of Bt cotton are

Long fibre and resistant to aphids
Medium yield, long fibre and resistant to beetle pests
high yield and production of toxic protein crystals which kill dipteran pests.
High yield and resistant to ball worms

Dicer and Drosha are __________

plasmids
rDNA
enzyme
transgenic plants

Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacterium is associated with the root nodules
of all cereals and pulse crops

Reason : A gene incorporated in the bacterial chromosomal genome gets automatically tra~sferred to the cross with
which bacterium is associated.

Both assertion and reason are true. But reason is correct explanation of assertion.
Both assertion and reason are true. But reason is not correct explanation of assertion.
Assertion is true, but reason is false.
Both assertion and reason are false.

Which one of the following is not correct statement.

Ti plasmid causes the bunchy top disease.
Multiple cloning site is known as Polylinker.
Non viral method transfection of Nucleic acid in cell.
Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

`In which techniques Ethidium Bromide is used?

Southern Blotting techniques
Western Blotting techniques
Polymerase Chain Reaction
Agrose Gel Electroporosis

The process of recombinant DNA technology has the following steps
I. amplication of the gene
II. Insertion of recombinant DNA into the host cells
III. Cutting of DNA at specific location using restriction enzyme .
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.

ii, iii, iv, and i
iv, ii, iii, and i
i, ii, iii, and iv
iv, iii, i, and ii

In which techniques Ethidium Bromide is used?

Southern Blotting techniques
Western Blotting techniques
Polymerase Chain Reaction
Agrose Gel Electroporosis

Consider the following statements:

I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the
manipulation of genetic materials by man in vitro.

II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid.

III. Restriction enzymes belongs to a class of enzymes called nucleases.

Choose the correct option regarding above statements

I&II
I & III
II&III
I,II & III

Who· discovered penicillin?

Alexander Flemming
Kohler
Milstein
Karl Ereky

DNA ligase is isolated from _____________

E-coli
TMV
T₄ phage
Agrobacterium

Flavr Savr tomato was created to _________

increase vitamin content
increase yield
increase shelf life
make tomatoes look brighter

_____________ used to produce algal biofuel.

Chlamydomonas reinhardii
Botryococcus braunii
Aequorea victoria
Alcaligenes eutrophus

Some of the charat:teristics of Bt cotton are

Long fibre and resistant to aphids.
Medium yield, long fibre and resistant to beetle pests.
high yield and production of toxic protein crystals which kill dipteran pests.
High yield and resistant to ball worms.

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