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12th Biology Weekly Test - 4 ( Botany - Plant Tissue Culture )

St. Britto Hr. Sec. School - Madurai

12th Biology Weekly Test - 4 ( Botany - Plant Tissue Culture )-Aug 2020

Time : 1.30 Hours

Part - A

Multiple Choice Question - Answer All Question

Name the phenomenon of the reversion of mature cells to the meristematic state.

Redifferentiation
Dedifferentiation
Totipotency
Differentiation

The prevention oflarge scale loss of biological integrity

Biopatent
Bioethics
Biosafety
Biofuel

Codeine is got from ____________

Catharanthus
Papaver
Digitalis
Cinchona

Shoot meristem tip culture is used to obtain virus-free plants because

shoot tip is got easily
shoot tip grows faster
shoot tip has the Apical bud
shoot meristem tip is always virus free

Cryopreservation means it is a process to preserve plant cells, tissues or organs

at very low temperature by using ether.
at very high temperature by using liquid nitrogen
at very low temperature of -196 by using liquid nitrogen
at very low temperature by using liquid nitrogen

Explant of ___________ sterile segment is selected from leaf for tissue culture.

1-3 cm
1-2 cm
1 -1.5 cm
1 - 4 cm

Virus free plants are developed from

Organ culture
Meristem culture
Protoplast culture
Cell suspension culture

Select the incorrect statement from given statement

A tonic used for cardiac arrest is obtained from Digitalis purpuria
Medicine used to treat Rheumatic pain is extracted from Capsicum annum
An anti malarial drug is isolated from Cinchona officinalis.
Anti-cancinogenic property is not seen in Catharanthus roseus.

Match the following :
Column A
Column B
1) Totipotency A) Reversion of mature cells into meristerm
2) Dedifferentiation B) Biochemical and structural changes of cells
3) Explant C) Properties of living cells develops into entire plant
4) Differentiation D) Selected plant tissue transferred to culture medium

C A D B
A C B D
B A D C
D B C A

Dimethyl sulphoxide is added during cryopreservation because it

reduces the temperature.
helps in enzymatic activities.
protects tissues from stress offreezing.
maintains tissues in dormant condition.

The time duration for sterilization process by using autoclave is minutes and the temperature is _

10 to 30 minutes and 125° C
15 to 30 minutes and 121° C
15 to 20 minutes and 125° C
10 to 20 minutes and 121° C

Cryopreservation means it is a process to preserve plant cells, tissues or organs

at very low temperature by using ether.
at very high temperature by using liquid nitrogen.
at very low temperature of -196 by using liquid nitrogen.
at very low temperature by using liquid nitrogen.

Micro propagation involves

vegetative multiplication of plants by using micro-organisms.
vegetative multiplication of plants by using small explants.
vegetative multiplication of plants by using microspores.
Non-vegetative multiplication of plants by using microspores and megaspores.

The prevention of large scale loss of biological interity

Biopatent
Bioethics
Biosafety
Biofuel

Select the incorrect statement from given statement.

A tonic used for cardiac arrest is obtained from Digitalis purpuria.
Medicine used to treat Rheumatic pain is extracted from Capsicum annum.
An anti malarial drug is isolated from Cinchona officinalis.
Anti-carcinogenic property is not seen in Catharanthus roseus.

Steam sterilization is done by autoclaving at

l20°C
121°C
115°C
151^oC

Match the following :

ColumnA ColumnB

(1) Totipotency (A) Reversion of mature
cells into meristerm

(2) Dedifferentiation (B) Biochemical and structural changes
of cells

(3) Explant (C) Properties of living cells develops into entire plant

(4) Differentiation (D) Selected plant tissue transferred to
culture medium

C A D B
A C B D
B A D C
D B D C

Which of the following statement is correct

Agar is not extracted from marine algae such as seaweeds.
Callus undergoes differentiation and produces somatic embryoids.
Surface sterilization of explants is done by using mercuric bromide
P^H of the culture medium is 5.0 to 6.0

Virus free plants are developed from

Organ culture
Meristem culture
Protoplast culture
Cell suspension culture

Vincristine is used as ____________

analgesic
cardiac tonic
anti carcinogenic substance
Rheumatic pain reliever

Artificial seeds are also called as

synthetic seeds
fibre seeds
mature seeds
Golden seeds

Which of the following statement is correct

Agar is not extracted from marine algae such as seaweeds
Callus undergoes differentiation and produces somatic embryoids.
Surface sterilization of explants is done by using mercuric bromide
P^H of the culture medium is 5.0 to 6.0

Solidifying agent used in plant tissue culture is

Nicotinic acid
Cobaltous chloride
EDTA
Agar

The embryoids are sub-cultured to produce ____________

plantlets
leaf
stem
root

Micro propagation involves

vegetative multiplication of plants by using micro-organisms.
vegetative multiplication of plants by using small explants.
vegetative multiplication of plants by using microspores.
Non-vegetative multiplication of plants by using microspores and megaspores.

Totipotency refers to

capacity to generate genetically identical plants.
capacity to generate a whole plant from any plant cell / explant.
capacity to generate hybrid protoplasts.
recovery of healthy plants from diseased plants.

The plant extracts are sterilized by passing through millipore filter with____________

0.2 mm pore diameter
0.4 mm pore diameter
0.2 mm pore diameter
0.3 mm pore diameter

_________ is a cryoprotectant.

glycerol
sodium alginate
macerozyme
MS medium

The time duration for sterilization process by using autoclave is minutes and the temperature is _

10 to 30 minutes and 125° C
15 to 30 minutes and 121° C
15 to 20 minutes and 125° C
10 to 20 minutes and 121° C

Solidifying agent used in plant tissue culture is

Nicotinic acid
Cobaltous chloride
EDTA
Agar

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