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12th Biology Monthly Test -2 ( Botany - Principles and Processes of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test -2 ( Botany - Principles and Processes of Biotechnology )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

Plasmids are

circular protein molecules
required by bacteria
tiny bacteria
confer resistance to antibiotics

The term biotechnology was coined by ___________

Gottlieb
W. Bateson
Karl Ereky
Erich

Which one of the following is not correct statement

Ti plasmid causes the bunchy top disease
Multiple cloning site is known as Polylinker
Non viral method transfection of Nucleic acid in cell
Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

In blue white colony selection method cells with non recombinant DNA develop ___________ colonies.

Blue
White
Blue with patches of white
Colourless

Genetic engineering is

making artificial genes.
hybridization of DNA of one organism to that. of the others.
production of alcohol by using micro organisms.
making artificial limbs, diagnostic instruments such as ECG, EEG etc.,

Consider the following statements:

I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the
manipulation of genetic materials by man in vitro.

II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid.

III. Restriction enzymes belongs to a class of enzymes called nucleases.

Choose the correct option regarding above statements

I&II
I & III
II&III
I,II & III

Who discovered restriction enzymes?

Watson and Crick
Avery-MacLeod-Mc Carty
Sanger and Gilbert
Arber, Smith and Nathans

Production of monoclonal antibodies by

Sanger and Gilbert
Kohler and Milstein
Watson and Crick
Karl Ereky

Match the following:

ColumnA ColumnB

1 Exonuclease (a) add or removephosphate

2 Endonuclease (b) binding the DNA fragments

3 Alkaline Phosphatase (c) cut the DNA at terminus

4 Ligase (d) cut the DNA at middle

a b c d
c d b a
a c b d
c d a b

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction
enzymes?

5' CGTTCG 3' 3' ATCGTA 5'
5' GATATG 3' 3' CTACTA 5'
5' GAATTC 3' 3' CTTAAG 5'
5' CACGTA 3' 3' CTCAOT 5'

Who discovered the double helix structure of DNA?

Sanger and Gilbert
Karl Ereky
Watson and Crick
Smith and Nathans

In which techniques Ethidium Bromide is used?

Southern Blotting techniques
Western Blotting techniques
Polymerase Chain Reaction
Agrose Gel Electroporosis

____________ used as biofertiliser and nitrogen fixers.

Enzymes
Biomass
Microbial inoculants
Biofuels

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?

5' CGTTCG 3' 3' ATCGTA 5'
5'GATATG 3' 3' CTACTA 5'
5'GAATTC 3' 3' CTTAAG 5'
5'CACGTA 3' 3' CTCAGT 5'

An analysis of chromosomal DNA using the southern hybridisation technique does not use

Electrophoresis
Blotting
Autoradiography
Polymerase Chain Reaction

Part - B

Generic 2 - Answer All Question

Comment on ELISA.

Name the two types of gene transfer methods in plants.

What are palindromic repeats?

Define Transfection.
Define plasmid.

Differentiate Symmetric and Asymmetric cuts of restriction enzymes.

What is LacZ?

What is the role of alkaline phosphatase in genetic engineering?

Part - C

Generic 3 - Answer All Question

Enumerate the applications of single-cell protein.

What do you know about the word pBR332?

Mention the application of Biotechnology.

Give an account of limitations of Bioremediation.

What are restriction enzyme. Mention their type with role in Biotechnology.

Explain the method of hydrogen production by algae.
Define Biopharming. Give its uses.

What are the materials used to grow microorganism like Spirulina?

Part - D

Generic 5 - Answer All Question

Explain the liposome mediated method of gene transfer?

Write the benefits and risk of Genetically Modified Foods.

What are single cell proteins? List out its uses?

Write the benefits and risk of Genetically Modified Foods.

Write the advantages of herbicide tolerant crops
Explain Southern blotting techniques.

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