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12th Biology Monthly Test - 2 ( Zoology - Molecular Genetics )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test - 2 ( Zoology - Molecular Genetics )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

Messelson and Stahl ___________.

proved that RNA is the genetic material
proved that protein synthesis is dependent on DNA
proved the semi conservative mode of DNA replication
discovered enzymes involved in replication

The distance between two consecutive base pairs in DNA is_________.

3.4 nm
0.34 nm
34.0nm
31x10^-9

A mRNA molecule is produced by

Replication
Transcription
Duplication
Translation

Wobble hypothesis ________.

proves that many tRNAs are. needed to transport specific amino acids
proves that the bases in tRNA need not match with mRNA
helps in economic usage of tRNA
proves that one tRNA can carry many amino acids

An operon is a:

Protein that suppresses gene expression
Protein that accelerates gene expression
Cluster of structural genes with related function
Gene that switched other genes on or off

E. coli cell grown on ¹⁵N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?

One high and one low density band
One intermediate density band.
One intermediate density band.
One low and one intermediate density band

_______demonstrated that RNA is the genetic material in RNA containing viruses.

Avery
Conrat and Singer
Griffith
Watson and Crick

The classical concept of a gene was given by_______ .

Mendel
Sutton
Johannsen
Hofmeister

The first codon to be deciphered was __ which codes for .

AAA, proline
GGG, alanine
UUU, Phenylalanine
TTT, arginine

One gene one enzyme hypothesis was proposed by Beadle and Tatum based on

Yeast
Drosophila
E. coli
Neurospora

An example of point mutation is __________.

Thalassemia
Diabetes
Turner syndrome
sickle cell anaemia

A nucleosome has _________ histone protein molecules.

6
8
10
24

What is the basis for the difference in the synthesis of the leading and lagging strand of DNA molecules?

Origin of replication occurs only at the 5' end of the molecules
DNA ligase works only in the 3' → 5' direction
DNA polymerase can join new nucleotides only to the 3' end of the growing stand.
Helicases and single-strand binding proteins that work at the 5' end

___________ used radioactive labelled molecules to prove that DNA is the genetic material.

Hershey and Chase
Wilkins and Franklin
Griffith
Mcleod and Avery

The concept of RNA world was independently proposed by __________

Orgel, Brick and Carl woese
Brick, Griffith and Crick
Wilkins and Franklin
Walter Gilbert

Part - B

Generic 2 - Answer All Question

Why is the term nucleic acid used for DNA and RNA?

State any three goals of the human genome project.

What is genophore?

What is a nucleosome?

What are nucleotides?

Give reasons: Genetic code is ‘universal’.

Differentiate - Template strand and coding strand.
Differentiate - Leading stand and lagging strand

Part - C

Generic 3 - Answer All Question

Distinguish between structural gene, regulatory gene and operator gene

What are the three structural differences between RNA and DNA?
A low level of expression of lac operon occurs at all the windows for treatment of various genetic disorders. Justify the statement

Define a operon.

What is reverse pharmacogenomicst?

State one gene one enzyme hypothesis

Why tRNA is called an adapter molecule?

What is a anticodon?

Part - D

Generic 5 - Answer All Question

It is established that RNA is the first genetic material. Justify giving reasons.

Explain the formation of a nucleosome.

Name the anticodon required to recognize the following codons: AAU, CGA, UAU, and GCA.

If the coding sequence in a transcription unit is written as follows:
5' TGCATGCATGCATGCATGCATGCATGC 3' Write down the sequence of mRNA

Why did Hershey and Chase use radioactively labelled phosphorous and sulphur only? Would they have got the same result if they use radiolabelled carbon and nitrogen?
How is the two stage process of protein synthesis advantageous?

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