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12th Biology Monthly Test - 2 ( Zoology - Applications of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test - 2 ( Zoology - Applications of Biotechnology )-Aug 2020

Time : 2.00 Hours

Part - A

Multiple Choice Question - Answer All Question

This is a live vaccine, in this genetically modified pathogens are made to non pathogenic.

Attenuated recombinant vaccine
Subunit recombinant vaccine
DNA vaccine
Traditional vaccine

The first synthetic vaccine produced was ________

DPT
HbsAg
Measles
Polio

Transgenic animals are those which have

Foreign DNA in some of their cells
Foreign DNA in all their cells
Foreign RNA in some of their cells
Foreign RNA in all their cells

Alpha lactalbumin is a protein with ______ aminoacids

120
133
123
140

Interferons were discovered by __________

Banting
Flemming
Engvall
Alick Isaacs and Lindemann

If a recessive gene is involved in causin genetic disease, what is the strategy to be u in gene therapy in order to cure the disease

Injecting DNA into the genome
Injecting DNA and antisense gene
Injecting antisense gene
Removal of recessive gene

Pre-pro insulin is devoid of __________

C chain
disulphide bonds
COOH and NH₂ group
Polypeptide chain

In the gene therapy far SCID,___________cells are removed from the blood, and ADA gene is introduced into it using retrovirus.

RBC
Wobe
Lymphocytes
monocytes

Best and Banting isolated insulin from pancreatic islets.of _____________

E.coli
yeast
dog
pig

The first clinical gene therapy was done for the treatment of

AIDS
Cancer
Cystic fibrosis
SCID

The two chains of Insulin molecule are attached by _______

Covalent bonds
disulphide bonds
hydrogen bonds
both a and b

The first clinical gene therapy was done for the treatment of

AIDS
Cancer
Cystic fibrosis
SCID

ELISA is mainly used for

Detection of mutations
Detection of pathogens
Selecting animals having desired traits
Selecting plants having desired traits

Interferons are produced using ___________

Pigs
yeast
E.coli
viruses

The genes for the formation of the blood clotting factor VIII is located in the ______________ chromosome.

autosome
X chromosome
allosome
Y chromosome

Part - B

Generic 2 - Answer All Question

What is GI?

Define pluripotency.

Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.

What is hGH?

Describe the chemical structure of insulin.
What is genetically engineered Insulin?

Define Biopiracy.

What is a patent?

Part - C

Generic 3 - Answer All Question

Name the strategies used in gene therapy.

Can interferons be isolated from blood.

Differentiate between Somatic cell gene therapy and germline gene therapy

What is the application of PCR in forensic science?

What is gene therapy? Mention the types.
What are stem cells? Explain its role in the field of medicine

What is HbsAg?

What are DNA vaccines?

Part - D

Generic 5 - Answer All Question

Explain the creation of Dolly.

PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate

Explain why cloning of Dolly, the sheep was such a major scientific breakthrough?

Mention the advantages and disadvantages of cloning.

List the uses of transgenesis.
Explain the steps involved in the production of recombinant hGH

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