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12th Biology Monthly Test - 2 ( Botany - Plant Tissue Culture )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test - 2 ( Botany - Plant Tissue Culture )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

Select the incorrect statement from given statement

A tonic used for cardiac arrest is obtained from Digitalis purpuria
Medicine used to treat Rheumatic pain is extracted from Capsicum annum
An anti malarial drug is isolated from Cinchona officinalis.
Anti-cancinogenic property is not seen in Catharanthus roseus.

The plant extracts are sterilized by passing through millipore filter with____________

0.2 mm pore diameter
0.4 mm pore diameter
0.2 mm pore diameter
0.3 mm pore diameter

Artificial seeds are also called as

synthetic seeds
fibre seeds
mature seeds
Golden seeds

The prevention of large scale loss of biological interity

Biopatent
Bioethics
Biosafety
Biofuel

Which of the following statement is correct

Agar is not extracted from marine algae such as seaweeds
Callus undergoes differentiation and produces somatic embryoids.
Surface sterilization of explants is done by using mercuric bromide
P^H of the culture medium is 5.0 to 6.0

Vincristine is used as ____________

analgesic
cardiac tonic
anti carcinogenic substance
Rheumatic pain reliever

Solidifying agent used in plant tissue culture is

Nicotinic acid
Cobaltous chloride
EDTA
Agar

Which of the following statement is correct

Agar is not extracted from marine algae such as seaweeds.
Callus undergoes differentiation and produces somatic embryoids.
Surface sterilization of explants is done by using mercuric bromide
P^H of the culture medium is 5.0 to 6.0

Micro propagation involves

vegetative multiplication of plants by using micro-organisms.
vegetative multiplication of plants by using small explants.
vegetative multiplication of plants by using microspores.
Non-vegetative multiplication of plants by using microspores and megaspores.

Micro propagation involves

vegetative multiplication of plants by using micro-organisms.
vegetative multiplication of plants by using small explants.
vegetative multiplication of plants by using microspores.
Non-vegetative multiplication of plants by using microspores and megaspores.

Solidifying agent used in plant tissue culture is

Nicotinic acid
Cobaltous chloride
EDTA
Agar

Totipotency refers to

capacity to generate genetically identical plants.
capacity to generate a whole plant from any plant cell / explant.
capacity to generate hybrid protoplasts.
recovery of healthy plants from diseased plants.

The time duration for sterilization process by using autoclave is minutes and the temperature is _

10 to 30 minutes and 125° C
15 to 30 minutes and 121° C
15 to 20 minutes and 125° C
10 to 20 minutes and 121° C

Match the following :
Column A
Column B
1) Totipotency A) Reversion of mature cells into meristerm
2) Dedifferentiation B) Biochemical and structural changes of cells
3) Explant C) Properties of living cells develops into entire plant
4) Differentiation D) Selected plant tissue transferred to culture medium

C A D B
A C B D
B A D C
D B C A

Shoot meristem tip culture is used to obtain virus-free plants because

shoot tip is got easily
shoot tip grows faster
shoot tip has the Apical bud
shoot meristem tip is always virus free

Part - B

Generic 2 - Answer All Question

Comment on Agar.

What is somatic embryogenesis?

Draw a sketch to show role of IPR in India.

Why is a sterile environment important in tissue culture?

Write the various steps involved in cell suspension culture.

Why is MS media used in plant tissue culture?
Comment on cybrid.

Give examples of two secondary metabolites produced by plants.

Part - C

Generic 3 - Answer All Question

How will you avoid the growing of microbes in nutrient medium during culture process? What
are the techniques used to remove the microbes?

What is Cryopreservation?

Differentiate Somadonal variations and Gametodonal variation.

What is organogenesis?

Explain the Biosafety guidelines are being implemented.
Write the various steps involved in cell suspension culture.

Give the examples for micro propagation performed plants .

What are the ethical issues in genomic research?

Part - D

Generic 5 - Answer All Question

Based on the material used, how will you classify the culture technology? Explain it.

Explain in detail about IPR.
Draw the flow chart of plant regeneration pathway in tissue culture.

Write the protocol for artificial seed preparation.

What do you know about Germ plasm conservationf Describe it.

Write the application of plant tissue culture.

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