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12th Biology Monthly Test -1 ( Zoology - Applications of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test -1 ( Zoology - Applications of Biotechnology )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

In the gene therapy far SCID,___________cells are removed from the blood, and ADA gene is introduced into it using retrovirus.

RBC
Wobe
Lymphocytes
monocytes

Early detection of the disease is not possible using their conventional diagnostic method.

microscopic examination
recombinant DNA technology
polymerase chain reactions
enzyme linked immunosorbent assay

Insulin was first isolated by ________

Banting
Allen
Wilmut
Anderson

Most of the adult stem cells are ________and can act as a repair system of the body.

Totipotent
Multipotent
Oligopotent
Pluvipotent

This type of gene therapy involves the insulin C the anti sense gene which inhibits the expression of the dominant gene.

gene inhibition therapy
gene augmentation therapy
germ line gene therapy
somatic cell gene therapy

Choose the option not applicable to ELISA,

It is a bio chemical produce to detect antigens in a sample.
The intensity of colour obtained in the test is proportional to amount of antigen.
It can detect DNA
ELISA is a test for detecting human chorionic gonadotropin hormone

Throgh PCR technique, amplication of__________ is possible with reverse transcriptase.

DNA
rRNA
tRNA
mRNA

The first clinical gene therapy was done for the treatment of

AIDS
Cancer
Cystic fibrosis
SCID

Pre-pro insulin is devoid of __________

C chain
disulphide bonds
COOH and NH₂ group
Polypeptide chain

Using recombinant E.Coli, mass production of hGH is carried out by ________

genetic engineering
biotechnology
fermentation technology
recombinant DNA

How many amino acids are arranged in the two chains of Insulin

Chain A has 12 and Chain B has 13
Chain A has 21 and Chain B has 30 amino acids
Chain A has 21 and Chain B has 30 amino acids
Chain A has 12 and chain B has 20 amino acids.

This is not the advantage of new generation vaccine.

They trigger immune response only against not specific pathogens.
They produce target proteins
They produce long lasting immunity
They are with less toxic effect.

If a recessive gene is involved in causin genetic disease, what is the strategy to be u in gene therapy in order to cure the disease

Injecting DNA into the genome
Injecting DNA and antisense gene
Injecting antisense gene
Removal of recessive gene

Interferons are produced using ___________

Pigs
yeast
E.coli
viruses

The first synthetic vaccine produced was ________

DPT
HbsAg
Measles
Polio

Part - B

Generic 2 - Answer All Question

What is BRAI?

What is a patent?

Name the types of ELISA test.

What is alpha lactalbumin?

Gene therapy is an attempt to correct a Genetic defect by providing a normal gene into the individual. By this the function can be restored. An alternate method would be to provide gene product known as enzyme replacement therapy, which would also restore the function. Which in your opinion is a better option? Give reasons for your answer.
Explain how “Rosie” is different from a normal cow

What is denaturation.?

What is hGH?

Define molecular cloning.

Define totipotency.

Name the types of recombinant vaccines.

What is bioethics?

Define pluripotency.

What is GI?

How is the amplification of a gene sample of interest carried out using PCR?

What does ELISA stands for?

Part - C

Generic 3 - Answer All Question

What is the application of PCR in forensic science?

What is HbsAg?
What are stem cells? Explain its role in the field of medicine

One of the applications of biotechnology is ‘gene therapy” to treat a person born with a hereditary disease
i) What does “gene therapy” mean?
ii) Name the hereditary disease for which the first clinical gene therapy was used.
iii) Mention the steps involved in gene therapy to treat this disease.

Can DNA vaccine cause the disease?

What is oligopotency?

What are the precautions to be taken in gene therapy?

What is gene augmentation therapy?

What is a stem cell bank?

Explain how ADA deficiency can be corrected

If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?

Part - D

Generic 5 - Answer All Question

Explain the creation of Dolly.
Mention the advantages and disadvantages of cloning.

Explain how recombinant Insulin can be produced.

Explain why cloning of Dolly, the sheep was such a major scientific breakthrough?

PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate

What are recombinant vaccines?. Explain the types

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