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12th Biology Monthly Test -1 ( Botany - Principles and Processes of Biotechnology )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test -1 ( Botany - Principles and Processes of Biotechnology )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

T₁ plasmid does not have this gene.

Onc
tra
ori
inc

Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.

Both assertion and reason are true. But reason is correct explanation of assertion.
Assertion is true, but reason is false.
Assertion is false, but reason is true
Both assertion and reason are false.

Who discovered restriction enzymes?

Watson and Crick
Avery-MacLeod-Mc Carty
Sanger and Gilbert
Arber, Smith and Nathans

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?

5' CGTTCG 3' 3' ATCGTA 5'
5'GATATG 3' 3' CTACTA 5'
5'GAATTC 3' 3' CTTAAG 5'
5'CACGTA 3' 3' CTCAGT 5'

_____________ used to produce algal biofuel.

Chlamydomonas reinhardii
Botryococcus braunii
Aequorea victoria
Alcaligenes eutrophus

Which one of the following is not correct statement.

Ti plasmid causes the bunchy top disease.
Multiple cloning site is known as Polylinker.
Non viral method transfection of Nucleic acid in cell.
Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

Who discovered the double helix structure of DNA?

Sanger and Gilbert
Karl Ereky
Watson and Crick
Smith and Nathans

Which of the following one is used as a Biosensors?

Electrophoresis
Enzymes
Electroporation
Bioreactors

Which of the following one is used as a Biosensors?

Electrophoresis
Bioreactors
Vectors
Electroporation

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction
enzymes?

5' CGTTCG 3' 3' ATCGTA 5'
5' GATATG 3' 3' CTACTA 5'
5' GAATTC 3' 3' CTTAAG 5'
5' CACGTA 3' 3' CTCAOT 5'

`In which techniques Ethidium Bromide is used?

Southern Blotting techniques
Western Blotting techniques
Polymerase Chain Reaction
Agrose Gel Electroporosis

Conventional biotechnology is also known as

Modern biotechnology
Traditional biotechnology
Old biotechnology
Kitchen biotechnology

_____________ is a ·reconstructed plasmid.

PBR 322 plasmid
Ti plasmid
Vectors
Jumping plasmid

An analysis of chromosomal DNA using the southern hybridisation technique does not use

Electrophoresis
Blotting
Autoradiography
Polymerase Chain Reaction

____________ used as biofertiliser and nitrogen fixers.

Enzymes
Biomass
Microbial inoculants
Biofuels

Part - B

Generic 2 - Answer All Question

What are cosmids?

Define plasmid.
Name the fungal organisms used as SCP.

What is meant by Transgenic plants?

What is meant by recombinant DNA technology?

Define Electrophoresis.

Comment on Lambda genome.

Differentiate Primary metabolites and Secondary metabolites.

What is meant by genome sequencing?

What is insert?

Name the basic tools which are required for rDNA technology.

What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

Name the two types of gene transfer methods in plants.

What are palindromic repeats?

What is biotechnology?

Comment on ELISA.

Part - C

Generic 3 - Answer All Question

How do you use the biotechnology in modern practice?

Write a note on bioprospecting.

How will you identify a vectors?

What do you know about the word pBR332?

What are the enzymes you can used to cut terminal end and internal phospho diester bond of nucleotide sequence?

What are the types of vectors?

Explain the method of hydrogen production by algae.
Differentiate Insertion vector and the Replacement vector.

Mention the application of Biotechnology.

Is their any possibilities to transfer a suitable desirable gene to host plant without vector? Justify your answer.

How can biotechnology used in plants for virus resistance?

Part - D

Generic 5 - Answer All Question

What are restriction enzyme. Mention their type with role in Biotechnology.

Compare the various types of Blotting techniques
Write about the process of fermentation.

Give an account of BASTA

Is their any possibilities to transfer a suitable desirable gene to host plant without vector? Justify your answer.

Define Biopiracy. Explain with Neem as an example?

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