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12th Biology Monthly Test -1 ( Botany - Plant Tissue Culture )

St. Britto Hr. Sec. School - Madurai

12th Biology Monthly Test -1 ( Botany - Plant Tissue Culture )-Aug 2020

Time : 2.30 Hours

Part - A

Multiple Choice Question - Answer All Question

The embryoids are sub-cultured to produce ____________

plantlets
leaf
stem
root

Dimethyl sulphoxide is added during cryopreservation because it

reduces the temperature.
helps in enzymatic activities.
protects tissues from stress offreezing.
maintains tissues in dormant condition.

Artificial seeds are also called as

synthetic seeds
fibre seeds
mature seeds
Golden seeds

The prevention of large scale loss of biological interity

Biopatent
Bioethics
Biosafety
Biofuel

Virus free plants are developed from

Organ culture
Meristem culture
Protoplast culture
Cell suspension culture

Match the following :
Column A
Column B
1) Totipotency A) Reversion of mature cells into meristerm
2) Dedifferentiation B) Biochemical and structural changes of cells
3) Explant C) Properties of living cells develops into entire plant
4) Differentiation D) Selected plant tissue transferred to culture medium

C A D B
A C B D
B A D C
D B C A

Which of the following statement is correct

Agar is not extracted from marine algae such as seaweeds.
Callus undergoes differentiation and produces somatic embryoids.
Surface sterilization of explants is done by using mercuric bromide
P^H of the culture medium is 5.0 to 6.0

Shoot meristem tip culture is used to obtain virus-free plants because

shoot tip is got easily
shoot tip grows faster
shoot tip has the Apical bud
shoot meristem tip is always virus free

Cryopreservation means it is a process to preserve plant cells, tissues or organs

at very low temperature by using ether.
at very high temperature by using liquid nitrogen
at very low temperature of -196 by using liquid nitrogen
at very low temperature by using liquid nitrogen

The time duration for sterilization process by using autoclave is minutes and the temperature is _

10 to 30 minutes and 125° C
15 to 30 minutes and 121° C
15 to 20 minutes and 125° C
10 to 20 minutes and 121° C

Name the phenomenon of the reversion of mature cells to the meristematic state.

Redifferentiation
Dedifferentiation
Totipotency
Differentiation

Select the incorrect statement from given statement.

A tonic used for cardiac arrest is obtained from Digitalis purpuria.
Medicine used to treat Rheumatic pain is extracted from Capsicum annum.
An anti malarial drug is isolated from Cinchona officinalis.
Anti-carcinogenic property is not seen in Catharanthus roseus.

Vincristine is used as ____________

analgesic
cardiac tonic
anti carcinogenic substance
Rheumatic pain reliever

Codeine is got from ____________

Catharanthus
Papaver
Digitalis
Cinchona

Totipotency refers to

capacity to generate genetically identical plants.
capacity to generate a whole plant from any plant cell / explant.
capacity to generate hybrid protoplasts.
recovery of healthy plants from diseased plants.

Part - B

Generic 2 - Answer All Question

What is Dedifferentiation?

What is the name of the process given below? Write its 4 types.

Draw a sketch to show role of IPR in India.

Who coined the term Totipotency?

What are secondary metabolites?

What is redifferentiation?

What is meant by Totipotency?

What do you mean Embryoids? Write its application.

Mention the media formulation available for plant tissue culture other than MS.

Give examples of two secondary metabolites produced by plants.

What is explant?
What are embryoids?

Differentiate Organ culture and Meristem culture.

What is PEG?

How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

What is meant by tissue culture?

Part - C

Generic 3 - Answer All Question

How will you avoid the growing of microbes in nutrient medium during culture process? What
are the techniques used to remove the microbes?

How are synthetic seeds / artificial seeds produced?

What is patents?

Give the examples for micro propagation performed plants .

What is Cryopreservation?
Differentiate Somadonal variations and Gametodonal variation.

Mention two potential risks involved in Biosafety.

Write the various steps involved in cell suspension culture.

Draw the flow chart of general steps in patenting.

Write a note on hardening technique in plant tissue culture.

Based on the material used, how will you classify the culture technology? Explain it.

Part - D

Generic 5 - Answer All Question

Based on the material used, how will you classify the culture technology? Explain it.

What do you know about Germplasm conservation. Describe it.

Give an account on Cryopreservation.

Write the protocol for artificial seed preparation.
Write the protocol for artificial seed preparation.

Highlight the protocol for production of virus free meristem tip culture.

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